The iNaturalist application has been gaining traction in both biodiversity research and education. Increasingly more research is being carried out, looking into using the iNaturalist application in biology education, to promote biodiversity and educate students about this topic. We used the PRISMA systematic literature review method to examine research carried out using the iNaturalist application in biology education by searching Web of Science and Scopus, and including citizen science in the process. We searched through the iNaturalist forum, where users contribute research performed using iNaturalist. We found that an increasing number of articles have been published on this topic over the years. Researchers are using iNaturalist to improve BioBlitz activities, to enhance outdoor education activities, and to help make herbaria. Summarizing the work performed in this area, we suggest guidelines for including and preparing the iNaturalist application in a school context to be used by teachers and researchers alike.